International Mammalian Genome Society

The 15th International Mouse Genome Conference (2001)

Table of Contents * Complex Genetics * Developmental Genetics * Gene Annotation * Gene Discovery * Genome Sequencing * Functional Genomics * Mutagenesis * Presentations * Verne Chapman Memorial Lecture

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POSTER 125 - APEROXIREDOXIN II INDUCES HEMOLYSIS AND INFERTILITY IN MICE

Dr Dae-Yeul Yu
Lab. of Animal Developmental Biotechnology
P.O.BOX 115, Yusong
Taejon
305-600
Republic of Korea

Co-Authors:  1) Lee TH, 1) Kim SU, 1) Yu SL, 1) Jeong S, 1) Han Y, 1) Moon HB, 2) Kim CK, 2) Shin HS, 1) Lee KK, 3) Rhee SG
Institutions: 1) School of Medicine, Wonkwang University, 2) Pohang University of Science and Technology,  3) NIH

Peroxiredoxin(Prx), which functions as anti-oxidant, in the protection of thiol groups, has been reported. To understand the biological significance of peroxiredoxin, which is relatively abundantly expressed in the adult as well as in developing embryos, we generated PrxII null mice. Phenotypical abnormalities have been observed.  Splenomegaly appeared in homozygote mice from 1 month of age and were peak during 4 to 5 month of age. Red pulp was markedly increased in the spleen of homozygote mice compared to that of wild mice. Hemosiderin staining shows spleen with iron overloaded. Precipitates of denatured globin (Heinz body) revealed in 30% of red blood cells from homozygote mice. These results suggest that PrxII may play a role in protecting against oxidative stress occurred in red blood cells during the transport of oxygen into tissues. Another phenotype appeared in PrxII null female mice is markedly reduced fertility. We are now in the process of identifying the reason.

Taken together we generated PrxII null mice exhibiting abnormality in Red blood cell and infertility due to oxidative stress generated by the deletion of PrxII.