International Mammalian Genome Society

The 16th International Mouse Genome Conference (2002)

Plenary Presentations * Oral Presentations *
Poster Presentations: Complex Genetics and Disease Modifiers * Developmental Genetics * Functional Genomics * Gene Discovery * Genetic Manipulations to Alter Gene Function * Mouse Models: Human Disease and Pharmacogenetics * Sequence Annotation and Comparative Analysis of Genomes *
Attendees * Sponsors * Table of Contents * Photographs * Awards

POSTER 8 - ANALYSIS OF IN VITRO RADIOSENSITIVITY OF MOUSE PERIPHERAL LEUKOCYTES BY THE MULTI-LANE COMET ASSAY

M Ohta
National Institute of Radiological Sciences

Goto M, Iwakawa M, Imai T, Harada Y
RadGenomics Project, Frontier Research Center, National Institute of Radiological Sciences

Clinical radiobiology has been stimulated by the search for predictive assays to determine the intrinsic tumor or normal cellular radiosensitivity of patients with aim to individualize clinical radiotherapy. In order to achieve this purpose, laboratory mice are very important to identify some genes which are related to the radiosensitivity. The comet assay is an excellent procedure to study DNA damage and its repair of eucaryote cells. Recently, the multi-lane comet assay method was originally developed with the aim of the high throughput comet assay. Our new method can analyse six samples simultaneously on one slide and also minimize experimental errors between slides observed in conventional comet assay.       The whole blood was collected from mice of four inbred strains (C3H/HeMs, C57BL/6J, A/J and STS/A) and of two congenic strains (C3H-scid and C.B.17-scid). These samples were irradiated with 0, 2, 4 and 8 Gy to analyse initial DNA damage. For DNA repair studies, the samples were incubated at 37 ºC after irradiation of 4 Gy and were analysed at 5, 15, and 30 minutes by multi-lane comet assay. For initial damage, significant differences of the tail moment (TM) were not observed in the mouse strains including the scid strains. In the repair study, TM values of the two scid strains were significantly higher than those of the other inbred strains. Interestingly, TM of the C3H-scid mice was higher than that of C.B.17-scid. Our data suggests that C3H mice have the gene(s) which is related to radiosensitivity by cooperating with the scid gene.