International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany

Plenary Presentations * Oral Presentations *
Poster Presentations: Behavioural Genetics and Genomics * Development and Stem Cells * Functional Genome Analysis * Mouse Models of Human Disease * Mouse System Biology Bioinformatics * Multigenic and Multifactorial Trait Analysis * Nutrition and Metabolic Disease * Phenotyping Methods Imaging * The Genetics and Genomics of Infectious Disease *
Verne Chapman Memorial Lecture * Table of Contents * Sponsor/Exhibitor List * Awards * Photographs

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POSTER 75 - ANALYSIS OF MCRII: A CONSERVED REGION OF THE WT1 ANTISENSE TRANSCRIPT

Allsop J
University of Bath

Co-Authors: 1) Moorwood K, 2) Brown K, 2) Malik K 1) Ward A
Institutions: 1) University of Bath, 2) University of Bristol

WT1 is a phylogenetically conserved nuclear protein essential for a variety of developmental processes affecting both transcriptional regulation and RNA metabolism. To understand the mechanisms of WT1 regulation, in vivo experiments are crucial, because the complex and strict developmental expression pattern of WT1 is not accounted for by the transcriptional regulatory elements reported from in vitro experiments. Previous studies indicate that human WT1 antisense transcripts (WT1-AS) can regulate WT1 and that WT1-AS is subject to developmental control via genomic imprinting. MCRII, a region of WT1-AS, is highly conserved between man and mouse strongly suggesting it has a regulatory role. MCRII has also been independently identified as an enhancer element in vivo. We are developing a mouse knockout model to test whether MCRII is a key regulator of Wt1 expression in development.