International Mammalian Genome Society

17th International Mouse Genome Conference

9-12 November 2003, Braunschweig, Germany

Plenary Presentations * Oral Presentations *
Poster Presentations: Behavioural Genetics and Genomics * Development and Stem Cells * Functional Genome Analysis * Mouse Models of Human Disease * Mouse System Biology Bioinformatics * Multigenic and Multifactorial Trait Analysis * Nutrition and Metabolic Disease * Phenotyping Methods Imaging * The Genetics and Genomics of Infectious Disease *
Verne Chapman Memorial Lecture * Table of Contents * Sponsor/Exhibitor List * Awards * Photographs

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POSTER 106 - AUTOMATED DNA PURIFICATION FROM MOUSE TAILS

Mulrooney C
Tepnel Life Sciences PLC

Co-Authors: Howe S, Oultram J, Robinson J, Maloney S, Sayle J
Institutions: Tepnel Life Sciences PLC

The extraction of high quality genomic DNA is the first and often limiting step in the process of mouse tail genotyping. Tail snips are difficult samples to work with and most of the chemistries and systems available on the market today fail to consistently produce sufficient DNA of an appropriate quality to meet the requirements of both PCR† and Southern blotting.

Tepnel's Nucleopure automation platform, using the patented Nucleon® chemistry, produces excellent yields of highly intact and pure DNA from tail snips and can process up to 192 samples in 2 ½ hours. The chemistry, plasticware, software and automation have all been integrated and optimised with this application in mind.